The Biogenesis of Peroxisomal Catalase in Rat Liver

Author

Paul Bernard Lazarow

Date of Award

1972

Document Type

Thesis

Degree Name

Doctor of Philosophy (PhD)

RU Laboratory

Bronk Laboratory

Abstract

Rat liver peroxisomal catalase was purified to homogeneity. The starting material was purified peroxisomes, The purity of the final preparation was judged by Sephadex G-200 chromatography, SDS gel electrophoresis, spectral analysis, and immunochemical means. Two sera containing anticatalase antibodies were prepared. The first, a rabbit serum, contained a minor impurity. The anticatalase was purified forty times by affinity chromatography and the contaminating antibodies were thereby removed. The second serum, prepared in a goat, was proved to be monospecific by immunodiffusion and immunoelectrophoresis.

Comments

A thesis submitted to the Faculty of the Rockefeller University in partial fulfillment of the requirement for the degree of Doctor of Philosophy

Recommended Citation

Lazarow, Paul Bernard, "The Biogenesis of Peroxisomal Catalase in Rat Liver" (1972). Student Theses and Dissertations. 545.
https://digitalcommons.rockefeller.edu/student_theses_and_dissertations/545