Student Theses and Dissertations

Author

Rufei Li

Date of Award

2024

Document Type

Thesis

Degree Name

Doctor of Philosophy (PhD)

RU Laboratory

Ruta Laboratory

Abstract

In many species, males have evolved elaborate behavioral patterns to attract females or repel rival males. The neural mechanisms mediating these male behavioral traits and how females use them to guide their mating decisions remain elusive. In this thesis,I present our efforts to uncover the neural logic underlying mate selection in Drosophila melanogaster.Unlike males who display overt courtship behaviors in pursuit of a female mate, including chasing her and singing a courtship song to her, female behaviors during courtship are subtle, making it challenging to infer how a female’s sexual receptivity fluctuates based on the male’s performance.To gain insight into dynamic changes in female sexual receptivity, we explored two distinct female behaviors displayed during courtship. First, we characterized the ‘avoidance’ behaviors displayed by females in response to pursuit by an inappropriate mate, where a fast approaching visual target elicits rapid turning and acceleration but can be suppressed by male courtship song. Neural circuits underlying avoidance behavior appear to interface with those governing a female’s sexual receptivity, providing an indirect reflection of her mating preferences. Second, we analyzed female vaginal plate opening, a final motor program indicative of her sexual acceptance of a male, and how it is elicited in response to male performance. We reveal that the probability that a male’s song triggers females to open their vaginal plates varies over time, which likely reflects dynamic fluctuations in a female’s sexual receptivity.

Comments

A Thesis Presented to the Faculty of The Rockefeller University in Partial Fulfillment of the Requirements for the degree of Doctor of Philosophy

Available for download on Wednesday, April 29, 2026

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