Student Theses and Dissertations

Adi Y. Berman

Date of Award

2024

Document Type

Thesis

Degree Name

Doctor of Philosophy (PhD)

RU Laboratory

Kapoor Laboratory

Abstract

The γ-Tubulin ring complex (γ-TuRC) is an essential regulator of the microtubule cytoskeleton. It is composed of>30 individual proteins that include the major component,γ-tubulin, as well as γ-tubulin complex proteins 2-6 (GCP2-6), mitotic-spindle organizing proteins associated with a ring of γ-tubulin proteins 1 and 2 (MOZART, or MZT1 andMZT2), and an actin molecule. This ~2.2MDa assembly regulates microtubule dynamics by facilitating the nucleation of new microtubules, modulating microtubule minus-end dynamics by acting as a minus-end cap, and anchoring microtubules to specify their cellular localization. These three major activities of the γ-TuRC, nucleation, capping, and anchoring, contribute to the dynamic nature of individual microtubules and of larger microtubule networks that are critical for cellular activities, including cell motility, intrace have spurred biochemical and cellular characterization of its nucleation activity. However,it remains unclear how the remaining major γ-TuRC activities contribute to microtubule dynamics.

Comments

A Thesis Presented to the Faculty of The Rockefeller University in Partial Fulfillment of the Requirements for the degree of Doctor of Philosophy

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