Student Theses and Dissertations

Date of Award


Document Type


Degree Name

Doctor of Philosophy (PhD)

RU Laboratory

Lipmann Laboratory


Sulfate activation and transfer to lipids, polysaccharides, and aminoalkyl phenols have been studied In a variety of biological systems. The transfer of S3504 from radioactive 3'-phosphoadenoslne 5'-phosphosulfate (PAPS35) into lipid-soluble material has been investigated in cell-free preparations of rat brain and liver. In brain of 10-20 day old rats activity was found in the particulate fraction of the homogenate, whereas in young adult rat liver activity resided in the supernatant fraction (105,000 x g). In both systems incorporation was stimulated by the addition of a crude mixture of the isomers of N-acetyl sphingosine. Evidence accumulated suggesting that the radioactive sulfolipids produced by these two system were probably different.


A thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at The Rockefeller Institute

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