Enzymatic Sulfurylation Mechanisms

Author

Irving Hyman Goldberg

Date of Award

1960

Document Type

Thesis

Degree Name

Doctor of Philosophy (PhD)

Thesis Advisor

Fritz Lipmann

Related Theses

View more theses from the Fritz Lipmann Laboratory

Keywords

sulfate activation, PAPS, sulfolipids, rat brain, rat liver, N-acetyl sphingosine

Abstract

Sulfate activation and transfer to lipids, polysaccharides, and aminoalkyl phenols have been studied In a variety of biological systems. The transfer of S³⁵O₄ from radioactive 3'-phosphoadenoslne 5'-phosphosulfate (PAPS³⁵) into lipid-soluble material has been investigated in cell-free preparations of rat brain and liver. In brain of 10-20 day old rats activity was found in the particulate fraction of the homogenate, whereas in young adult rat liver activity resided in the supernatant fraction (105,000 x g). In both systems incorporation was stimulated by the addition of a crude mixture of the isomers of N-acetyl sphingosine. Evidence accumulated suggesting that the radioactive sulfolipids produced by these two system were probably different.

Comments

A thesis presented to the faculty of The Rockefeller University in partial fulfillment of the requirements for the degree of Doctor of Philosophy

License and Reuse Information

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Recommended Citation

Goldberg, Irving Hyman, "Enzymatic Sulfurylation Mechanisms" (1960). Student Theses and Dissertations. 581.
https://digitalcommons.rockefeller.edu/student_theses_and_dissertations/581