Comparison of the Protein Contents of Bovine Zymogen Granules and of Pancreatic Juice

Author

Lewis Joel Greene

Date of Award

1962

Document Type

Thesis

Degree Name

Doctor of Philosophy (PhD)

Abstract

A zymogen granule fraction has been isolated from bovine pancreas by differential centrifugation under conditions designed to favor homogeneity at the expense of yield. The fraction has been examined in the electron microscope and found to contain a limited amount of contamination represented by clearly recognizable microsomes and mitochondria. These contaminants could account for a part of the 5% phospholipid and 1% nucleic acid found in the fraction by Schneider partition. The zymogen granule fraction was composed mainly of protein, about 95%. The protein content of the granules has been solubilized by exposure to pH 8, after which a membrane fraction could be isolated by centrifugation. This fraction is assumed to represent the smooth surfaced membranes which bound in situ the zymogen granules.

Comments

A thesis submitted to the Faculty of The Rockefeller Institute in partial fulfillment of the requirements for the degree of Doctor of Philosophy

Recommended Citation

Greene, Lewis Joel, "Comparison of the Protein Contents of Bovine Zymogen Granules and of Pancreatic Juice" (1962). Student Theses and Dissertations. 577.
https://digitalcommons.rockefeller.edu/student_theses_and_dissertations/577