Date of Award
Doctor of Philosophy (PhD)
The order of addition of amino acids to a growing protein is determined by the codon sequence of a messenger RNA molecule. This translation process was studied in vitro with a cell-free protein synthesis system derived from Escherichia coli. The rate of protein synthesis was proportional to the amount of messenger RNA added to the system. However, it was observed that different messenger RNA's were not equally effective in promoting protein synthesis. Experiments were conducted to determine why the rate of protein synthesis depends on the type of messenger RNA.
Kramer, Fred Russell, "Factors Affecting Translation of Messenger RNA's In Vitro: Use of a GTP Analogue to Investigate Rates of Polypeptide Chain Elongation" (1969). Student Theses and Dissertations. 564.