Factors Affecting Translation of Messenger RNA's In Vitro: Use of a GTP Analogue to Investigate Rates of Polypeptide Chain Elongation

Author

Fred Russell Kramer

Date of Award

1969

Document Type

Thesis

Degree Name

Doctor of Philosophy (PhD)

Thesis Advisor

Vincent Allfrey

Related Theses

View more theses from the Vincent Allfrey Laboratory

Abstract

The order of addition of amino acids to a growing protein is determined by the codon sequence of a messenger RNA molecule. This translation process was studied in vitro with a cell-free protein synthesis system derived from Escherichia coli. The rate of protein synthesis was proportional to the amount of messenger RNA added to the system. However, it was observed that different messenger RNA's were not equally effective in promoting protein synthesis. Experiments were conducted to determine why the rate of protein synthesis depends on the type of messenger RNA.

Comments

A thesis presented to the faculty of The Rockefeller University in partial fulfillment of the requirements for the degree of Doctor of Philosophy

License and Reuse Information

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Recommended Citation

Kramer, Fred Russell, "Factors Affecting Translation of Messenger RNA's In Vitro: Use of a GTP Analogue to Investigate Rates of Polypeptide Chain Elongation" (1969). Student Theses and Dissertations. 564.
https://digitalcommons.rockefeller.edu/student_theses_and_dissertations/564