Nuclear and Plasma Membrane Properties of Macrophage Heterokaryons and Hybrids

Author

Siamon Gordon

Date of Award

1971

Document Type

Thesis

Degree Name

Doctor of Philosophy (PhD)

RU Laboratory

Cohn Laboratory

Abstract

Mouse peritoneal macrophages, which do not synthesize DNA in vitro, were fused with a strain of mouse melanoma cells which proliferates rapidly in vitro. The plasma membrane of the macrophage has specific receptors which enable the cell to ingest antibodycoated sheep red cells and is also rich in a divalent cation dependent adenosine triphosphatase (ATPase) activity. The melanoma cell lacks these macrophage membrane markers. High yields of mouse macrophage-melanoma cell heterokaryons and macrophage-macrophage homokaryons were obtained through the Sendai virus -induced fusion of cells spread on a glass surface. After fusion there was a striking reorganization of cellular architecture by means of a colcemid-sensitive proces s. Heterokaryons were isolated through the use of differential trypsinization and many underwent division to form melanoma-like hybrids. The selective uptake of dextran sulfate by macrophages served as a useful cytoplasmic marker in identifying hybrids.

Comments

A thesis submitted to the Faculty of The Rockefeller University in partial fulfillment of the requirements for the degree of Doctor of Philosophy

Recommended Citation

Gordon, Siamon, "Nuclear and Plasma Membrane Properties of Macrophage Heterokaryons and Hybrids" (1971). Student Theses and Dissertations. 547.
https://digitalcommons.rockefeller.edu/student_theses_and_dissertations/547