Characterization of Phagosome-Lysosome Fusion in Macrophages

Author

Margaret Carol Kielian

Date of Award

1981

Document Type

Thesis

Degree Name

Doctor of Philosophy (PhD)

RU Laboratory

Cohn Laboratory

Abstract

Several approaches were used to study the determinants of phagosome-lysosome {P-L} fusion in intact mouse macrophages. Lysosomes were labeled with the fluorescent vital dye acridine orange and the rate and extent of their fusion with yeast-containing phagosomes was monitored by fluorescence microscopy. Fusion was also assayed by electron microscopy, using horseradish peroxidase or thorium dioxide as markers for secondary lysosomes. Good agreement was found with results obtained from vitally-stained cells, thin section samples using an enzymatic marker, and thorium dioxide-labeled samples evaluated by stereology.

Comments

A thesis submitted to the Faculty of The Rockefeller University in partial fulfillment of the requirements for the degree of Doctor of Philosophy

Recommended Citation

Kielian, Margaret Carol, "Characterization of Phagosome-Lysosome Fusion in Macrophages" (1981). Student Theses and Dissertations. 478.
https://digitalcommons.rockefeller.edu/student_theses_and_dissertations/478