On the Isolation of Oöcytase, the Fertilizing and Cytolyzing Substance in Mammalian Blood-Sera

Authors

T. Brailsford Robertson

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Description

T. Brailsford Robertson. On the isolation of oöcytase, the fertilizing and cytolyzing substance in mammalian blood-sera, 1912

It has been observed by Loeb that the eggs of sea-urchins (Strongylocentrotus purpuratus) may be induced to form fertilization-membranes by immersing them in mammalian blood-sera which have been rendered isotonic with sea-water by the addition of NaCl. Occasionally, if the serum be especially potent, simple immersion of the eggs in the serum suffices to bring about this result, but as a rule previous treatment of the eggs with a sensitizing agent (SrCl₂ or CaCl₂) is required. Prolonged action of the serum upon sensitized eggs results in the cytolysis of the eggs. Very potent sera usually cause agglutination of the eggs as well, especially of sensitized eggs.

Full text

Publication Date

1912

Publisher

Cornell Medical College

City

New York

Keywords

T. Brailsford Robertson

Recommended Citation

Robertson, T. Brailsford, "On the Isolation of Oöcytase, the Fertilizing and Cytolyzing Substance in Mammalian Blood-Sera" (1912). Pamphlets, Offprints and Reprints. 21.
https://digitalcommons.rockefeller.edu/pamphlets-offprints-and-reprints/21