Date of Award
Doctor of Philosophy (PhD)
A number of studies have demonstrated that small metabolites can react nonenzymatically with proteins to form covalent, addition products. An example of this type of reaction 1s the modification of hemoglobin A by glucose to form the minor species hemoglobin A1c. In this case, glucose reacts by forming a reversible Schiff base adduct with the N-terminal valine of the β -chain of hemoglobin. The Schiff base intermediate then slowly undergoes an Amadori rearrangement to produce a stable ketoamine addition product.
Bucala, Richard J., "The Nonenzymatic Modification of Proteins by Steroids and DNA by Reducing Sugars; In Vitro and In Vivo Studies" (1984). Student Theses and Dissertations. 585.