Date of Award
Doctor of Philosophy (PhD)
An alternating copolymer of deoxy 2,6 diamino purine and thymine (dDAP-T) has been synthesized by substituting 2,6 diamino purine deoxyriboside triphosphate for dATP in a dAT primed synthesis with DNA polymerase. This polymer differs from dAT in having an amino group in the minor groove, and in several physical properties. For example, the additional amino group allows a third hydrogen bond of the Watson-Crick type to be formed with thymine, and the expected increase in the stability of the helix is reflected in a transition temperature (Tm) which is higher by 25oC. than that of dAT.
Cerami, Anthony, "Biochemical Studies of the Purine Analogues, 2-Aminopurine and 2, 6 Diaminopurine" (1967). Student Theses and Dissertations. 568.