Date of Award
Doctor of Philosophy (PhD)
Amino acids are activated as aminoacyladenylates which remain bound to the aminoacyl-tRNA synthetases that catalyze their formation. The activated amino acids are then esterified to specific transfer RNA molecules. By this reaction sequence, the specificity and energetics necessary for polypeptide synthesis are conferred upon the amino acids. The first method of determining acyl group activation was the hydroxamate assay. The activated amino acids can be trapped as the stable amino acid hydroxamates by carrying out the activation reactions in high concentrations of hydroxylamine.
Hirsch, David Ian, "The Hydroxamate Reaction of Aminoacyl-tRNA Synthetases" (1968). Student Theses and Dissertations. 566.