Date of Award
Doctor of Philosophy (PhD)
The studies reported in this thesis describe the conditions elucidated for the selective reductive carboxymethylation of the a-amino termini of hemoglobin (Hb)1 and the structural and functional consequences of such a modification. The initial premise for such a modification (HbNHCH2COO- was to test its usefulness as a carbon dioxide (CO2) or carbamino analogue (HbNHCOO- ). The latter compound is formed reversibly (which is physiologically necessary) and cannot be isolated. The former is irreversibly formed and, when prepared in sufficient amounts, could lead to a wealth of information concerning the binding of CO2 to Hb as well as the interplay between this effector and other important physiological modulators of hemoglobin.
Fantl, Wendy Jane, "The Specific Carboxymethylation of the N-Terminal Amino Groups of Human Hemoglobin: Structural and Functional Implications" (1986). Student Theses and Dissertations. 480.