Student Theses and Dissertations

Date of Award


Document Type


Degree Name

Doctor of Philosophy (PhD)


The studies reported in this thesis describe the conditions elucidated for the selective reductive carboxymethylation of the a-amino termini of hemoglobin (Hb)1 and the structural and functional consequences of such a modification. The initial premise for such a modification (HbNHCH2COO- was to test its usefulness as a carbon dioxide (CO2) or carbamino analogue (HbNHCOO- ). The latter compound is formed reversibly (which is physiologically necessary) and cannot be isolated. The former is irreversibly formed and, when prepared in sufficient amounts, could lead to a wealth of information concerning the binding of CO2 to Hb as well as the interplay between this effector and other important physiological modulators of hemoglobin.


A thesis submitted to the faculty of The Rockefeller University in partial fulfillment of the requirements for the degree of Doctor of Philosophy

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