Induction of Innate and Adaptive Immunity In Vivo by Selective Antibody Targeting of Poly dA:dT and Antigen to Dendritic Cells

Author

Scott A. Barbuto

Date of Award

2012

Document Type

Thesis

Degree Name

Doctor of Philosophy (PhD)

RU Laboratory

Steinman Laboratory

Abstract

Dendritic cells (DCs) have been shown to be efficient and specialized in antigen presentation, but innate stimuli have not been selectively delivered to determine if these cells alone can integrate innate and adaptive immunity in vivo. Here we illustrate a novel method using expressed protein ligation (EPL) to attach the immune stimulant, poly dA:dT, to the DC uptake receptor, DEC205 (DEC). We will show that inoculation of α- DEC-poly dA:dT fusion monoclonal antibodies (mAbs) is sufficient to induce rapid innate cytokine production by DCs. Adaptive T cell immunity is also elicited when the fusion mAb is given in combination with a DC-targeted antigen. Forced entry is not required because α-DEC fusion mAbs deliver the poly dA:dT to its cytosolic targets.

Comments

A Thesis Presented to the Faculty of The Rockefeller University in Partial Fulfillment of the Requirements for the degree of Doctor of Philosophy

Recommended Citation

Barbuto, Scott A., "Induction of Innate and Adaptive Immunity In Vivo by Selective Antibody Targeting of Poly dA:dT and Antigen to Dendritic Cells" (2012). Student Theses and Dissertations. 243.
https://digitalcommons.rockefeller.edu/student_theses_and_dissertations/243