CD4 antigen, ymphoblastoid cell line, dendritic cell, lymphocyte clone, T lymphocyte
The accessory cell requirements for cloning T cells in the presence of lectin and T cell growth factors were examined with cells from human peripheral blood. We found that dendritic cells were active and perhaps essential. Single CD4+ lymphocytes could be cloned with 80% efficiency, and CD8+ cells with 50-60% efficiency if 103 syngeneic or allogeneic dendritic cells were added. Some T cell clones developed even with one dendritic cell. Monocytes or B lymphocytes from blood were at least 100-fold weaker in supporting clonal growth. These findings suggest a specialized feeder cell requirement, namely dendritic cells, for cloning T lymphocytes from single resting precursors.
Langhoff, E. and R. M. Steinman. 1989. "Clonal Expansion of Human T Lymphocytes Initiated by Dendritic Cells." Journal of Experimental Medicine 169 (1): 315-320