On the Isolation of Oöcytase, the Fertilizing and Cytolyzing Substance in Mammalian Blood-Sera
T. Brailsford Robertson. On the isolation of oöcytase, the fertilizing and cytolyzing substance in mammalian blood-sera, 1912
It has been observed by Loeb that the eggs of sea-urchins (Strongylocentrotus purpuratus) may be induced to form fertilization-membranes by immersing them in mammalian blood-sera which have been rendered isotonic with sea-water by the addition of NaCl. Occasionally, if the serum be especially potent, simple immersion of the eggs in the serum suffices to bring about this result, but as a rule previous treatment of the eggs with a sensitizing agent (SrCl2 or CaCl2) is required. Prolonged action of the serum upon sensitized eggs results in the cytolysis of the eggs. Very potent sera usually cause agglutination of the eggs as well, especially of sensitized eggs.
Cornell Medical College
T. Brailsford Robertson
Robertson, T. Brailsford, "On the Isolation of Oöcytase, the Fertilizing and Cytolyzing Substance in Mammalian Blood-Sera" (1912). Pamphlets, Offprints and Reprints. 21.